Professional Experience:
Research:
Our laboratory aims to unravel pathogenesis induced by important zoonotic pathogens and develop intervention strategies. We established the steller role of virulence-plasmid in Aeromonas hydrophila-pathogenesis and successfully used plasmid-cured isolates as vaccine candidates against its spread. MDR-A. hydrophila is global issue. We sequenced Ah-virulence-plasmid, demonstrating quinolone and beta-lactamase resistance is plasmid-encoded, opening new avenues in controlling the bacterium. Our group reported the role of calcium and its dependent kinases in macrophage apoptosis induced by zoonotic pathogens, and established the importance of ER-mitochondrial Ca-dynamics in the process. We demonstrated how zoonotic-pathogens exploit TLR4-topology for immune-evasion and persistence inside the host. The strikingly similar mechanisms of cell death induced in fish and mammals clearly suggested that pathogenic mechanisms employed by zoonotic-pathogens transcend species barrier. Toxoplasma gondii resides perpetually inside infected host. Work emanating from our laboratory described how the parasite manipulates β-catenin signaling and usurps tryptophan-metabolites for intracellular survival, suggesting alternate approach for its treatment.
Xenobiotic-immunotoxicity has been the other major focus of my group. We mapped the course of events leading to profound immunosuppression caused by arsenic and identifed the signature genes involved in arsenic-induced apoptosis.These findings are important from the viewpoint of predictive toxicology and biomarker development. Recently, we elucidated that chronic endosulfan-exposure cause immune commotion wherein the immune-organs per se, generate endosulfan-metabolites, principal components of endosulfan-toxicity, thereby compounding the toxic effects of the pesticide. Fluorosis is a major health issue and our work on fluoride-induced immunotoxicity has contributed significantly towards the wellness of the society.
3. Kumar M, Sharma S, Haque M, Kumar J, Hathi UPS, Mazumder S. (2022) TLR22- Induced Pro-Apoptotic mtROS abets UPRmt-mediated mitochondrial fission in Aeromonas hydrophila-infected headkidney macrophages of Clarias gariepinus. Frontiers in Immunology. DOI: 10.3389/fimmu.2022.931021
4. Kumar J, Kumar M, Sharma S, Srivastava N, Singh R, Hussain MA, Mazumder S. (2022). Th1–Th2 and M1-M2 interplay sculpt Aeromonas hydrophila pathogenesis in zebrafish (Danio rerio). Fish & Shellfish Immunology. DOI:10.1016/j.fsi.2022.06.052
5. Kumar M, Shelly A, Dahiya P, Ray A, Mazumder S. (2022) Aeromonas hydrophila inhibits autophagy triggering cytosolic translocation of mtDNA which activates the pro-apoptotic caspase-1/IL-1β-nitric oxide axis in headkidney macrophages. Virulence. 2022. DOI: 10.1080/21505594.2021.2018767
Dahiya. P, Hussain, Md. A, Mazumder. S. (2021). mtROS Induced via TLR-2-SOCE Signaling Plays Proapoptotic and Bactericidal Role in Mycobacterium fortuitum-Infected Head Kidney Macrophages of Clarias gariepinus. Front Immunol. 12:748758. DOI:10.3389/fimmu.2021.748758
Kumar. M, Kumar. J, Sharma. S, Hussain, Md, A., Shelly, A., Das. B, Yadav. A.K, Mazumder. S. (2021). TLR22-mediated activation of TNF-α-caspase-1/IL-1β inflammatory axis leads to apoptosis of Aeromonas hydrophila-infected macrophages. Mol. Immunol. Doi: 10.1016/j.molimm.2021.06.025
Hussain. Md. Arafat, Datta. D, Singh. R, Kumar. M, Kumar.J, Mazumder. S. (2019). TLR-2 mediated cytosolic-Ca2+ surge activates ER-stress-superoxide-NO signalosome augmenting TNF-α production leading to apoptosis of Mycobacterium smegmatis infected fish macrophages. Sci Rep. DOI: 10.1038/s41598-019-48847-1
Majumdar. T, Sharma. S, Kumar. M, Hussain. Md. A, Chauhan. N, Kalia. I, Sahu, A.K, Rana. V.S, Bharti. R, Haldar. A.K, Singh. A.P, Mazumder. S. (2019). Tryptophan kynurenine pathway attenuates β-catenin-dependent pro-parasitic role of STINGTICAM2-IRF3-IDO1 signalosome in Toxoplasma gondii infection. Cell Death Disease DOI:10.1038/s41419-019-1420-9
Datta. D, Khatri. P, Singh. A, Saha. D.R. Verma. G, Raman. R, Mazumder. S. (2018). Mycobacterium fortuitum-induced ER-Mitochondrial calcium dynamics promotes calpain/caspase-12/caspase-9 mediated apoptosis in fish macrophages. Cell Death Discov. DOI: 10.1038/s41420-018-0034-9
Srivastava. N, Shelly. A, Kumar. M, Pant. A, Das. B, Majumdar.T, Mazumder. S. (2017). Aeromonas hydrophila utilizes TLR4 topology for synchronous activation of MyD88 and TRIF to orchestrate anti-inflammatory responses in zebrafish. Cell Death Discov. 3. 17067. DOI: 10.1038/cddiscovery.2017.09.006
Ray. A, Bhaduri. A, Srivastava. N, Mazumder. S. (2017). Identification of novel signature genes attesting arsenic-induced immune alterations in adult zebrafish (Danio rerio). J Hazard Mater. DOI: 10.1016/j.jhazmat.2016.09.001
Banerjee. C, Singh. A, Das. T.K, Rajagopal. R, Srivastava. A, and Mazumder. S (2014). Ameliorating ER-stress attenuates Aeromonas hydrophila-induced mitochondrial dysfunctioning and caspase mediated HKM apoptosis in Clarias batrachus. Sci Rep. DOI: 10.1038/srep05820
Banerjee. C, Khatri. P, Rajagopal. R, Bhatia. H, Datta. M and Mazumder, S. (2014). Role of Calmodulin-Calmodulin Kinase II, cAMP/Protein Kinase A and ERK 1/2 on Aeromonas hydrophila-Induced Apoptosis of Head Kidney Macrophages. PLOSPathogens. 10.1371/journal.ppat.1004018